NF-kB p65 antibody (Biotin)

Catalog No. ABIN7697277

The Rabbit Polyclonal anti-NF-kB p65 antibody has been validated for WB, IHC and ICC. It is suitable to detect NF-kB p65 in samples from Mouse.

Quick Overview for NF-kB p65 antibody (Biotin) (ABIN7697277)

Target: NF-kB p65 (NFkBP65) (Nuclear Factor-kB p65 (NFkBP65))

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NF-kB p65 antibody is conjugated to Biotin

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC)

Product Details anti-NF-kB p65 Antibody (Biotin)

Purpose: Biotin-Linked Polyclonal Antibody to Transcription Factor P65 (NFKB3)

Specificity: The antibody is a rabbit polyclonal antibody raised against NFKB3. It has been selected for its ability to recognize NFKB3 in immunohistochemical staining and western blotting.

Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Isotype: IgG

Application Details

Application Notes: Western blotting: 0.2-2 μg/mL,1:250-2500 Immunohistochemistry: 5-20 μg/mL,1:25-100 Immunocytochemistry: 5-20 μg/mL,1:25-100 Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 500 μg/mL

Buffer: PBS, pH 7.4, containing 0.02 % Sodium azide, 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Target Details for NF-kB p65

Target: NF-kB p65 (NFkBP65) (Nuclear Factor-kB p65 (NFkBP65))

Alternative Name: Transcription Factor P65

Background: Nuclear factor NF-kappa-B p65 subunit, NFKBP65, RELA, p65, Nuclear Factor Of Kappa Light Polypeptide Gene Enhancer In B-Cells 3

UniProt: Q04207

Pathways: NF-kappaB Signaling, RTK Signaling, TCR Signaling, TLR Signaling, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Hepatitis C, Toll-Like Receptors Cascades, S100 Proteins